Alteration in Rhizosphere Soil Properties of Afforested <Emphasis Type="Italic">Rhamnus lycioides</Emphasis> Seedlings in Short-Term Response to Mycorrhizal Inoculation with <Emphasis Type="Italic">Glomus intraradices</Emphasis> and Organic Amendment

The reestablisment of autochthonous plant species is an essential strategy for recovering degraded areas under semiarid conditions. A field experiment was carried out to assess the short-term effect of two reafforestation methods involving mycorrhizal inoculation and compost addition on soil quality parameters and Rhamnus lycioides seedling growth. The nutrient content (NPK) and enzymatic activities (dehydrogenase, urease, protease-BAA, acid phosphatase and β-glucosidase) increased and bulk density decreased in the rhizosphere soil with the organic amendment. Biomass C of rhizosphere soil increased by at least 240% with respect to the control soil after mycorrhizal inoculation and the combination of compost addition + mycorrhizal inoculation. Both mycorrhizal inoculation and composted organic residue addition increased R. lycioides seedling growth in the same proportion. In the short term, we conclude that the application of both reafforestation methods not only enhances the establishment of R. lycioides seedlings, but also improves soil quality.     

The impact of impression‐management tactics on supervisor ratings of organizational citizenship behavior

Previous research indicates that the use of impression management is related to supervisor ratings of employee likeability and job performance. It has been argued, though, that employees might also use impression management so that their superiors will see them as ‘good soldiers’ who engage in high levels of organizational citizenship behaviors (OCBs). This study investigates the ways in which three types of impression management—namely, job‐focused, self‐focused, and supervisor‐focused tactics of impression management—influence supervisor ratings of OCB. Using a sample of 122 supervisor‐subordinate dyads, the findings indicate that supervisor‐focused tactics of impression management are positively related to OCB ratings, while job‐focused tactics are negatively related to such evaluations. Additionally, citizenship behaviors are positively related to supervisor liking of the employee and overall ratings of job performance. Finally, the results suggest that OCB ratings mediate the relationship between supervisor‐focused tactics of impression management and supervisor evaluations of employee likeability. Some implications of this study and directions for future research are also addressed. Copyright © 2006 John Wiley & Sons, Ltd.     

Effect of Cross-Linking Waste Protein with Diepoxides on its Biodegradation under Anaerobic Conditions

Biodegradability testing was performed in an aqueous environment under anaerobic conditions after inoculation with digested sludge from municipal wastewater treatment plant. In cross-linking with 1,2:3,4-diepoxybutane in limits 0.8–9.1% weight, biodegradability degree decreased from 76.8 to 62.2%; when 1,2:7,8 diepoxyoctane in quantities 1.1–13.2% weight was used, biodegradability degree dropped more prominently – from 72.3 to 22.8%. There is obviously a direct connection between growing cross-link degree (assessed by so-called fixation index) and decreasing readiness to biodegradation, apparently owing to build-up of a network forming an obstacle to access of micro-organisms and enzymes.     

Study of Pollution of the Plitvice Lakes by Water and Sediment Analyses

The process of eutrophication in form of intense plant growth has been observed in some lakes and water streams at the Plitvice Lakes National Park in central Croatia. Here we investigate whether this phenomenon is a consequence of anthropogenic pollution or due to naturally produced organic matter in the lakes. We applied chemical analysis of water at two springs and four lakes (nutrients, dissolved organic carbon (DOC), trace elements) and measurements of surface lake sediments (mineral and organic fraction analyses, trace elements) in four different lakes/five sites. The chemical composition of water does not indicate recent anthropogenic pollution of water because the concentrations of most trace elements are below detection limits. The concentrations of DOC and nutrients are slightly higher in the area of increased eutrophication-plant growth. Also the content of organic matter in the sediment is at the highest level in areas with highest C/N ratio indicating that the organic fraction of this sediment is mainly of terrestrial origin. There is no significant difference among the trace element concentration in the upper segment of all cores, deposited approximately during last 50 years when higher anthropogenic influence is expected due to development and touristic activity, and the lower part of the cores, corresponding to the period approximately 100–200 years before present. The content of trace elements and organic matter in sediments decreases from the uppermost lake downstream. According to our results there is no indication of recent anthropogenic pollution in water and sediment. Higher concentrations of DOC in water as well as phosphorus and some other elements in the lake sediment can be a consequence of input of natural organic matter to the lake water.     

Equilibrium and kinetic studies on biosorption of Hg(II), Cd(II) and Pb(II) ions onto microalgae Chlamydomonas reinhardtii

The microalgae Chlamydomonas reinhardtii was used for the biosorption of Hg(II), Cd(II) and Pb(II) ions. The maximum adsorption of Hg(II) and Cd(II) ions on Chlamydomonas reinhardtii biomass was observed at pH 6.0 and the corresponding value for Pb(II) ions was 5.0. The biosorption of Hg(II), Cd(II) and Pb(II) ions by microalgae biomass increased as the initial concentration of Hg(II), Cd(II) and Pb(II) ions increased in the biosorption medium. The maximum biosorption capacities of microalgae for Hg(II), Cd(II) and Pb(II) ions were 72.2+/-0.67, 42.6+/-0.54 and 96.3+/-0.86 mg/g dry biomass, respectively. The affinity order for algal biomass was Pb(II)>Hg(II)>Cd(II). FT-IR analysis of algal biomass revealed the presence of amino, carboxyl, hydroxyl and carbonyl groups, which were responsible for biosorption of metal ions. Biosorption equilibrium was established in about 60 min and the equilibrium was well described by the Freundlich biosorption isotherms. Temperature change in the range of 5-35 degrees C did not affect the biosorption capacity. The microalgae could be regenerated using 0.1 M HCl, with up to 98% recovery, which allowed the reuse of the biomass in six biosorption-desorption cycles without any considerable loss of biosorption capacity.     

Biological treatment of clogged emitters in a drip irrigation system

This study was conducted to investigate microbial organisms that can be used for preventing clogging in drip irrigation systems caused by biological factors. A total of 25 fungi isolate and 121 bacterial strains were isolated from water samples collected from drip irrigation systems in tomato greenhouses in the eastern Anatolia region of Turkey in the spring season of 2001. Biological clogging of emitters in a model drip irrigation system was experimentally caused by application of the microorganisms (fungi and bacteria) isolated in the study. Three antagonistic bacterial strains in the genus Bacillus spp (ERZ, OSU-142) and Burkholdria spp (OSU-7) were used for treatment of biological clogging of the emitters. The results showed that the antagonistic bacterial strains tested have the potential to be used as anti-clogging agents for treatment of emitters in drip irrigation system. This is the first study that demonstrated that antagonistic microorganisms can be utilized for treatment of clogging in drip irrigation systems.     

Rapid metal extractability tests from polluted mining soils by ultrasound probe sonication and microwave-assisted extraction systems

Ultrasonic probe sonication (UPS) and microwave-assisted extraction (MAE) were used for rapid single extraction of Cd, Cr, Cu, Ni, Pb, and Zn from soils polluted by former mining activities (Mónica Mine, Bustarviejo, NW Madrid, Spain), using 0.01 mol L^?1 calcium chloride (CaCl₂), 0.43 mol L^?1 acetic acid (CH₃COOH), and 0.05 mol L^?1 ethylenediaminetetraacetic acid (EDTA) at pH 7 as extracting agents. The optimum extraction conditions by UPS consisted of an extraction time of 2 min for both CaCl₂ and EDTA extractions and 15 min for CH₃COOH extraction, at 30% ultrasound (US) amplitude, whereas in the case of MAE, they consisted of 5 min at 50 °C for both CaCl₂ and EDTA extractions and 15 min at 120 °C for CH₃COOH extraction. Extractable concentrations were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). The proposed methods were compared with a reduced version of the corresponding single extraction procedures proposed by the Standards, Measurements and Testing Programme (SM&T). The results obtained showed a great variability on extraction percentages, depending on the metal, the total concentration level and the soil sample, reaching high values in some areas. However, the correlation analysis showed that total concentration is the most relevant factor for element extractability in these soil samples. From the results obtained, the application of the accelerated extraction procedures, such as MAE and UPS, could be considered a useful approach to evaluate rapidly the extractability of the metals studied.     

Enrichment of trace elements in the clay size fraction of mining soils

Reactive waste dumps with sulfide minerals promote acid mine drainage (AMD), which results in water and soil contamination by metals and metalloids. In these systems, contamination is regulated by many factors, such as mineralogical composition of soil and the presence of sorption sites on specific mineral phases. So, the present study dedicates itself to understanding the distribution of trace elements in different size fractions (<2-mm and <2-μm fractions) of mining soils and to evaluate the relationship between chemical and mineralogical composition. Cerdeirinha and Penedono, located in Portugal, were the waste dumps under study. The results revealed that the two waste dumps have high degree of contamination by metals and arsenic and that these elements are concentrated in the clay size fraction. Hence, the higher degree of contamination by toxic elements, especially arsenic in Penedono as well as the role of clay minerals, jarosite, and goethite in retaining trace elements has management implications. Such information must be carefully thought in the rehabilitation projects to be planned for both waste dumps.     

Metal contents of marine turtle eggs (Chelonia mydas; Lepidochelys olivacea) from the tropical eastern pacific and the implications for human health

Concentrations of eight elements were measured in Chelonia mydas and Lepidochelys olivacea eggs collected along the Pacific coast of Panama. Manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), arsenic (As), cadmium (Cd), and mercury (Hg) concentrations were similar to previous reports of these species from around the world, while lead (Pb) was lower than previous reports. Cd posed the highest health risk to people who regularly eat the eggs, with average consumption rates leading to target hazard quotients (THQ) of up to 0.35 ± 0.15. Our conclusions indicate that current turtle egg consumption in isolated, coastal Pacific communities may pose a health concern for young children, and that youth and young adults should limit their consumption of turtle eggs to reduce their total intake of nonessential metals.